Cat. No: P2701, 66 rxn/30μl reaction
P2702, 330 rxn/30μl reaction
P2703, 3333 rxn/30μl reaction
P2704, 6666 rxn/30μl reaction
Multiplex Probe qPCR Mix Plus U
Components
Component
|
P2701
|
P2702
|
P2703
|
P2704
|
2× Multiplex Probe qPCR Mix Plus U
a
|
1ml
|
1 ml × 5
|
50 ml
|
100 ml
|
Nuclease-free Water
|
1
ml
|
1 ml × 5
|
-
|
-
|
a contains Hotstart Taq DNA Polymerase, dUTP/UDG, dNTP mix and reaction buffer.
Storage
This reagent can be stored for 2 months at 4°C. For longer storage, it should be kept at -20°C.
Description
Multiplex Probe qPCR Mix Plus U is a 2× concentrated premix for real-time quantitative PCR with
probe method. In use, just add the DNA template, primer and probe to react. This product contains
antibody technology modified Hotstart Taq DNA polymerase. Combined with GDSBio's special
real-time PCR Buffer, it can not only effectively inhibit primers dimer and other non-specific
amplification, but also improve the amplification efficiency, allowing multi-probe qPCR reaction. This
reagent introduced dUTP/UDG anti-contamination system, which can remove PCR products
containing dUTP before PCR reaction, effectively avoid the influence of cross contamination of
amplification products on quantification. This product is convenient for preparation of IVD molecular
diagnostic kit. The target gene quantification is accurate, reliable and reproducible. This product can
be used with TaqMan and other fluorescent probes, and is perfectly compatible with common
quantitative PCR instruments, such as ABI, Roche, Bio-rad, etc.
Applications
• Probe g
ene expression analysis
• Probe
Low-copy gene detection
• Probe m
icroarray validation
• Probe
gene knockdown validation
Features
• This kit is suitable for fluorescence quantification by probe method
• This kit is compatible with many real-time systems
• Hot-start technology brings high specificity and reproducible amplification