The Renji Monkeypox Virus Real Time PCR Kit is based on real-time PCR technology. Specific primers and probes are designed based on F3L gene areas of the Monkeypox virus. In addition, the kit also contains a housekeeping gene (RNase P) as an internal control (IC) for specimen sampling and nucleic acid extraction. The Monkeypox Virus Real Time PCR Kit applies to MA-6000, ABI series, Bio-Rad series, Roche LightCycler R480, Cepheid SmartCycler, Rotor-Gene series and other multi-channel real-time quantitative PCR instruments in the market.

Easy to use:  3-in-1 single-tube design, ready for testing after dispensing and loading.

High sensitivity:  ensured accuracy with the LoD of 200 copies/mL.

Automation ready:  compatible with Tianlong's automated nucleic acid extractors, for minimized manual errors.

Optimized efficiency:  rapid detection completed within 40min working with real-time PCR systems.

1. Reagent Preparation(performed at "reagent preparation region")
1.1 Take out each componentsfrom the diagnostic kitand placethemat room temperature. Allow thereagents to equilibrateatroom temperature, then vortex each of themrespectively forlater use.
1.2 According to thequantityof test specimens, MPV Positive Control, MPVNegative Control,and Internal control (IC), pipetteappropriate quantity of MPVPCR Mix andMPVEnzymeMix ( MPV PCR Mix 15 μl/test +MPV EnzymeMix 4 μl/test +Internal control (IC) 1 μl/test), mix them thoroughlytomake a PCR-Mastermix, centrifuge itinstantaneously forlater use.

1.3 Transfer the above-prepared reagents to the"specimen processing region" for later use.

2. Processing and loading ofspecimens (performed at "specimenprocessing region")
2.1 Thisdiagnostic kit does not include Viral RNA&DNA Extraction Kit. Itisrecommended to use Viral RNA&DNA ExtractionKit produced by Changsha Renji Medical Equipment Co., Ltd. to extract viral DNA. The specific operation is in accordancewith its instructions.
2.2 Add 20μl PCR-Mastermix into PCR reaction tube with 5μl above processed sample, MPV Positive Control and MPV NegativeControl, and cap the tube. Carry outfluorescence quantitativePCR detection on fluorescencePCR instrument.

3.PCRAmplification (performed at "nucleic acidamplificationarea")
3.1 Place PCRreaction tubes into thespecimenwells of the amplification equipment. SetupMPVPositive Control, MPV NegativeControl and specimens to be tested in the corresponding sequence and input specimen name.
3.2 Set cycle parameters according to the following table for PCR amplification.

Changsha Renji Medical Equipment Co.,Ltd   is a medical supply company specilizing in  products series, such as disposable virus specimen collection sampler and  Saliva collection kits for PCR test. Vacuum blood collection tubes and other medical consumables.   We are direct manufacturer   based on Changsha City Hunan Province. And many of our staffs are gene experts and professional doctors who have plenty of experience in this industry. Our products have been approved by many certificates. Such as   CE, SGS,ISO   and so on.